Of these, 16 EV biomarkers that are putatively associated with SCA either as positive or negative biomarkers passed the quality control steps and were used: VEGFA, GLUT1, HIF1α, MUC6, p53, pan-Cytokeratin, VEGF/PIGF, Vimentin, Inhibin, Chromogranin A, VEGFC, Carbonic Anhydrase IX, VHL, Calponin 1, Glycogen, and Cytokeratin 18. Here, HIF1A is linked to autosomal dominant cerebellar ataxia.