Importantly, the same as in human tumors, the majority of KRAS mutations identified in canine tumors are located at mutational hotspot G12, including G12A (detected in 2 melanomas), G12V (in 2 mammary carcinomas), G12D (in 1 mammary carcinoma and 1 squamous cell carcinoma), and G12C (in 1 melanoma) (Table S5). This evidence concerns the gene KRAS and squamous cell carcinoma.