Since there is endogenous MG53 expression in colorectal cancer cell HCT116, we established a primary colorectal cancer model induced by azoxymethane/dextran sodium sulfate (AOM/DSS) treatment using MG53 overexpressing (MG53-TG) or deficient (MG53-KO) mice (Supplementary Fig. 5a) as well as their corresponding wild type littermates as controls. Here, TRIM72 is linked to colorectal cancer.