Pathway enrichment analysis of the essential genes using the ‘Genetic and Chemical Perturbation database’51 showed that they are enriched in class 2 hepatoblastoma genes (CAIRO_HEPATOBLASTOMA_CLASSES_UP, n = 612), and are targets of BMP2, DREAM complex, MYC and β-catenin (Fig. 6d). This evidence concerns the gene BMP2 and hepatoblastoma.