DAGLA and hepatocellular carcinoma: To elucidate the underlying mechanism of the DAGLA/2-AG axis in HCC progression, GSEA was performed to verify the GO analysis results of RNA-seq data and revealed that gene sets encoding epithelial–mesenchymal transition, cell migration, liver cancer proliferation and the cell cycle were notably downregulated in Hep3B-DAGLA-KD cells (Fig. 6A–C).