In addition, deprivation of the anaplerotic amino acid glutamine in MYCN-amplified neuroblastoma cells, triggers apoptosis [48], highlighting a glutamine addiction necessary to replenish tricarboxylic acid (TCA) cycle intermediates that sustain cell viability, and consistent with the finding of augmented de novo glutamine synthesis in MYCN-amplified neuroblastoma cells [45]. Here, MYCN is linked to neuroblastoma.