The results of western blotting showed that the expression of Wnt/β‐catenin signalling pathway‐related proteins, including β‐catenin, c‐Myc, cyclin D1, CD44 and Met, was significantly lower in glioma cells treated with NSC‐CM than in those treated with the control medium (Fig. 5A,B). Here, CCND1 is linked to central nervous system cancer.