In these cells, infection with CHIKV at a multiplicity of infection (MOI) of 1 led to reduced CHIKV nsP3 protein expression (Fig. 3a to c) and an approximately 2-log10 reduction in viral titer compared to mock vector-transfected infected cells at 48 h p.i. (Fig. 3d). This evidence concerns the gene SH2D3C and infection.