Subsequent multivariate analysis of the 11 significant factors in the univariate analysis showed that the independent prognostic factors affecting PFS in CRC patients were age (HR=1.318, 95%CI=1.076–1.615, p=0.008), creatinine/cystatin C ratio (HR=1.286, 95%CI = 1.007–1.642, p=0.044), T stage (HR=1.559, 95%CI =1.173–2.073, p=0.002), N stage (p<0.001), M stage (HR=3.628, 95%CI=2.814–4.677, p<0.001) and CEA (HR=1.286, 95%CI=1.007–1.642, p=0.003) (Table 3). This evidence concerns the gene CST3 and colorectal carcinoma.