Consequently, it was determined that the anti-obesity effect of the crude saponins in mice fed a high fat diet including the reduction of blood triacylglycerol may be due to the inhibition of intestinal absorption of dietary fat by platycodin D. In a different study, 10 known triterpenoidal saponins were purified from Platycodi Radix, among them; platycodin A, C, D, and deapioplatycodin D, as all of them showed intestinal absorption inhibition of dietary fats mediated by pancreatic lipase inhibition (Xu et al., 2005). This evidence concerns the gene PNLIP and obesity due to melanocortin 4 receptor deficiency.