To study the effect of HMSN-ISO@ProA-PD-L1 Ab in vivo, Hepa1-6 cells (2 × 106) were injected under the skin of the right hind leg of wild-type C57BL/6 mice, and drug treatment was started when the tumor grew to 50–100 mm3 (Fig. 8A). The gene discussed is CD274; the disease is neoplasm.