Immunofluorescence assay with FITC-ghost pencil cyclic peptide and Comas Brilliant Blue staining showed that when TOP2A was knocked down, tumor cells exhibited contracted and disordered stress fibers, while when TOP2A was overexpressed, the cytoskeleton of tumor cells was remodeled and expression was enhanced (Fig. 7B,C). This evidence concerns the gene TOP2A and neoplasm.