Both the gating strategy and PMA and ionomycin stimulation controls were shown in supplementary Fig. S2A. After the CMS5 tumor rechallenge, CD8+ T cells produced more IFNγ and TNFα than those before the tumor rechallenge or in the CMS5 inoculated control group (Fig. 2B, supplementary Fig. S2B). Here, IFNG is linked to neoplasm.