Both the gating strategy and PMA and ionomycin stimulation controls were shown in supplementary Fig. S2A. After the CMS5 tumor rechallenge, CD8+ T cells produced more IFNγ and TNFα than those before the tumor rechallenge or in the CMS5 inoculated control group (Fig. 2B, supplementary Fig. S2B). This evidence concerns the gene CD8A and neoplasm.