To identify which of the 80 TSGs mediate the UHRF1 phenotype in KRAS mutant cancer cells, we designed a focused CRISPR library targeting those genes and used this library to perform a screen in Cas9-expressing A549 cells treated with either a non-targeting control sgRNA or an UHRF1-targeting sgRNA (Fig. 5a, Supplementary Fig. 5a, b, and Supplementary Data 9). The gene discussed is UHRF1; the disease is cancer.