Finally, we evaluated the correlation between ROSig and cancer marker pathways (HALLMARK set) and showed that ROSig was positively correlated with cell cycle-related pathways and metabolic-related pathways and negatively correlated with signaling pathways such as TGF-β, interferon, IL6-JAK-STAT3 and MYC (Figure 7E). The gene discussed is TGFB1; the disease is cancer.