demonstrated that upregulation of miR-214 significantly reduced the radioresistance of osteosarcoma cells, while upregulation of miR-214 increased its targeted binding to the 3’-UTR region of PHLDA2, resulting in decreased PHLDA2 expression and enhanced the radiosensitivity of osteosarcoma cells and a mouse xenograft model. This evidence concerns the gene PHLDA2 and osteosarcoma.