A comparison of tumor tissues from patients with osteosarcoma and the corresponding paracancerous tissues revealed that miR-106 (163), miR-130a (56), miR-21 (166), miR-95-3p (168), miR-92a (170), miR-1908 (60), and miR-148a (171), miR-373 (173), miR-532-5p (176), miR-128 (177) and other PI3K/AKT pathway-related microRNAs all showed high expression in osteosarcoma, and then the clinical and pathological data of these patients were analyzed and studied in depth, and their expression was correlated with clinical stage and TNM stage were positively correlated. The gene discussed is PIK3CA; the disease is osteosarcoma.