It was found that by overexpressing miR-101 and miR-139 (Figure 6D) can downregulate rock1 while causing PI3K/AKT and JAK/STAT signaling pathway inactivation, ultimately leading to the malignant behavior of osteosarcoma cells can be partially suppressed, including biological behaviors such as abnormal proliferation, migration and invasion (191, 197). This evidence concerns the gene PIK3CA and osteosarcoma.