We found that KD of CHCHD6 and CHCHD3, as well as all other MICOS subunits and SAMM50, significantly reduced their proliferation in an EdU incorporation assay (Figure 6—figure supplement 2A,B), supporting a potential link for CHCHD6 and other MICOS subunits in HLHS pathogenesis. This evidence concerns the gene SAMM50 and hypoplastic left heart syndrome.