The immunofluorescence results showed that although the micelle treatment group loaded with PTX (sAcP) and the micelle treatment group loaded with aPD-1 (sAMc) could both increase CD8+T cell infiltration, the sAMcP combination treatment group induce the most CD8+T cell to infiltrate the tumor, which was the main reason for the strongest inhibition of tumor growth in this group. This evidence concerns the gene CD8A and neoplasm.