Zeb-Gel was prepared by directly loading Zeb into hydrogels for inducing melanoma cell antigen production, and the results showed that the enhanced expression of melanoma antigen family E1 (MAGE-E1), tyrosinase-related protein-1 (TRP1), and melanoma cell adhesion molecule (CD146), along with a similar upregulation of PD-L1 expression in tumor cells, suggesting that the use of DNA methylation inhibitors enhances the immunogenicity of tumors and thus activates more T cells into TME. This evidence concerns the gene CD274 and neoplasm.