In TME, when tumor cells with high PD-L1 expression interconnected with PD-1+NKs, Th1-type transcription factors of NKs, NKs activation markers (CD69, CD38, CD56), upstream activation molecules (ZAP70, CD247), cytolytic molecules (GZMA, GZMK) and NKs activation cytokine receptors (IL2RB, IL12RB1, IL18RAP) were all downregulated, indicating that NKs activity was inhibited [36]. This evidence concerns the gene CD274 and neoplasm.