To further clarify whether MMA regulated Wnt signaling pathway, we verified the expression of the key point β-catenin and downstream protein p-GSK-3β by western blot assay, and the results showed that β-catenin increased while p-GSK-3β (Ser9) decreased only in MMA induced CRC cells (Fig. 4C–F). Here, GSK3B is linked to colorectal carcinoma.