Here, a large panel of samples from five EU countries was assembled and tested using two different IGRA kits, the ID Screen® Ruminant IFN-g (IDvet) and the BovigamTM TB Kit (Bovigam), in order to i) evaluate the performance of IGRAs under different epidemiological conditions in bovine tuberculosis-free herds with a view to assess its usefulness for granting and maintenance of the OTF status of herds and the intra-Community trade of animals, and ii) assess the impact of different cut-off values in both kits. The gene discussed is IFNG; the disease is Tuberculosis, Bovine.