In the European Union (EU), based on Annex III to Delegated Regulation (EU) 2020/689 and Annex I to Delegated Regulation (EU) 2020/688 of the Regulation (UE) 2016/429, the intradermal tuberculin tests and interferon (IFN)-γ release assay (IGRA) are the official tests for granting and maintenance of the official TB-free (OTF) herd status and to obtain the certification for intra-Community trade of animals. Here, IFNG is linked to tuberculosis.