Next, we found that the frequency of early activation marker CD69 on CD8+ T cells was decreased when treated with T-96 or Tofa in the presence of IL-2 for 24 h, and there was a dim collective effect but statistically insignificant when two molecules co-pretreated from both patients with vitiligo (Fig. 2E) and healthy individuals (Additional file 1: Fig. S3D). This evidence concerns the gene CD69 and vitiligo.