Subsequently, we evaluated the effector function of T-96 in CD8+ T cells and revealed that T-96 decreased the high frequencies of pro-inflammatory cytokine IFN-γ, as well as cytotoxic-associated GzmB and PRF like the effect of Tofa in both the patients and healthy individuals, and joint administration of both demonstrated mild synergistic action in vitiligo patients (Fig. 2F), but statistically insignificant in healthy controls (Additional file 1: Fig. S3E). The gene discussed is GZMB; the disease is vitiligo.