Here, we applied the OzFAD pipeline to characterize the total fatty acid profiles of three cancer cell lines (i.e., MCF7, LNCaP and LNCaP_SCD1i; SCD-1 inhibition: A939572) and investigate the effect of enzyme expression and inhibition of stearyl-CoA desaturase-1 (SCD-1/Δ9-desaturase) (Supplementary Note 7). The gene discussed is SCD; the disease is cancer.