In a model of cisplatin-induced nephrotoxicity, blockade of TRPA1 alleviated apoptosis, reduced the levels of IL-1β, IL-6, TNF-α, and interferon (IFN)-γ, decreased the levels of cleaved caspase-3, cleaved poly (ADP-ribose) polymerase PARP, and inducible nitric oxide synthase (iNOS), reduced the expression of p-IKKβ, p-JNK, p-ERK and p-p38, and enhanced the expression of IκBα.195 TRPA1 expression was increased in renal tubular epithelial cells from patients with acute kidney injury. This evidence concerns the gene NOS2 and acute kidney injury.