The results showed that peppermint oil, activated TRPM8 and the treated mice showed improvements in macroscopic and microscopic parameters and reduced weight loss during colitis.575 In addition, Peiris et al. observed that the use of TRPM8 agonist icilin for irritable bowel syndrome resulted in reduced release of inflammatory cytokines IL-1β, IL-6, and TNF-α in patient biopsies.580 These results suggest that specific activation of TRPM8 may alleviate experimental colitis and irritable bowel syndrome and may be a promising additional therapy for the treatment of IBD. This evidence concerns the gene TNF and colitis.