Irrespective of whether the fibrils formed under the conditions used here are identical to those found in AD brain or not, we believe that the methods and data we present here pave the way for unprecedented opportunities to dissect the molecular and sequence determinants of Tau aggregation and pathology formation and bring us closer to reproducing the structural diversity of Tau fibrils isolated from different Tauopathies. This evidence concerns the gene MAPT and tauopathy.