SHBG and cancer: Therewith, SHBG protein has been shown to reverse the pro-survival effects of estradiol in the MCF-7 cancer cell line after 48 h incubation [46]; however, in our study, the treatment of SHBG-silenced ASCs with the exogenous protein did not restore the basal apoptosis level; this discrepancy may be explained by the differences in treatment times, as 24 h incubation maybe insufficient for detectable significant changes in the apoptotic tendency of silenced ASCs.