Immunostaining indicated that the number of c‐Fos (a widely used indicator of cell activation)‐positive neurons was significantly decreased in the rACC (Figure 3B) as well as in the DRN (Figure 3F) compared with the sham group, suggesting that the rACC and DRN are both involved in comorbidities of chronic neuropathic pain and anxiety‐like behaviors without influencing the total CaMKIIα+ neurons (Figure 3C) or 5‐HT+ neurons (Figure 3G). This evidence concerns the gene FOS and Pain.