TNF and acute myeloid leukemia: An ELISA assay to measure TNF-α secretion in AML cell lines following CIGB-300 treatment corroborated microarray and qPCR data showing a cell line-specific regulation with elevated TNF-α expression in HL-60 (66.7 pg/mL) compared to OCI-AML3 (10.3 pg/mL) at 3 h of treatment (Fig. 7B).