Our review designated that AB wild-type zebrafish, Casper transparent mutants, transgenic Tg(fli1:EGFP) or crossbreeding of the above-mentioned strains, orthotopically transplanted at 48 hpf with 50–100 U87 cells to study GBM angiogenesis, U251 cells to study GBM proliferation, or PDX to achieve clinical relevance in high density and low infusion volume (nL) gradually acclimatized to 32–33 °C could comprise a successful, conducive and reproducible zebrafish GBM model. Here, FLI1 is linked to glioblastoma.