To determine whether the γ-globin −175 A>G edit could be accomplished in bone marrow–repopulating HSCs, healthy donor CD34+ HSPCs were electroporated with ABE7.10 or ABE8e RNP and transplanted into non-obese diabetic/severe combined immunodeficiency/IL-2Rγ−/−/KitW41/W41 (NBSGW) mice that underwent necropsy after 16–18 weeks. Here, IL2RG is linked to severe combined immunodeficiency.