Our study suggested that METTL5 expression in HCC specimens had a close link with kinase networks, such as myosin light chain kinase (MLCK), aurora kinase B (AURKB), ribosomal protein S6 kinase A4 (RPS6KA4) functioned on modulating mitosis, cell cycle checkpoint, DNA damage response, cell growth, and cell proliferation31,32. Here, RPS6KA4 is linked to hepatocellular carcinoma.