LPS, an abundant component in gut microbiome, is involved in CRC progression and metastasis.[40] Our ELISA results showed the increased serum LPS level in male mice, which was consistent with the report on the increased LPS level in patients with CRC and colonic hyperpermeability.[41] Moreover, our results displayed wider paracellular gap and lower protein expression of ZO‐1, Occludin, and Claudin‐3 (gut barrier function‐related markers) in male mice than female mice, suggesting more severe impairment of gut barrier function in male mice relative to female mice. Here, OCLN is linked to colorectal carcinoma.