In contrast, defining mutations of AML-MR in WHO5th were overall rare (range: 0.1–2.1%), frequently co-occurred with other defining alterations (e.g., EZH2 in PICALM::MLLT10), and could be found in various clusters rather than as a distinct group (Fig.S3A, F), leading to its exclusion as a defining category for pAML. Here, EZH2 is linked to acute myeloid leukemia.