We have generated a collection of 108 genetically barcoded protein kinase null mutants in A. fumigatus and, using Bar-seq mediated competitive fitness profiling reveal a critical role for the dual-specificity tyrosine-regulated kinase (DYRK) YakA in adaptation to iron limitation, temperature, pH, hypoxia and azole drug stress and in contrast to the role played by its orthologue in yeast-type fungi, pathogenicity in a murine model of infection. This evidence concerns the gene WEE1 and infection.