For this, we used CRISPR/Cas9 mediated gene editing to knock out VPS35L from liver cancer Huh-7 cells and then stably reconstituted VPS35L expression using an empty vector (EV), or VPS35L variants, including wild-type (WT), W6D, S829E, G902E, and G325E (Extended Data Fig. 3A). The gene discussed is VPS35L; the disease is liver cancer.