Then, we chose gamma-interferon (IFN-γ) (Yin et al., 2018) and tumor necrosis factor-α (TNF-α) (Fu et al., 2012) related to ovarian dysfunction for the model of premature ovarian failure to detect their gene expression, as well as the expression levels of luteinizing hormone (LH), follicle-stimulating hormone (FSH), estrogen (E2) (Keremu et al., 2019), and anti-mullerian hormone (AMH) (Massin et al., 2008), which are features of premature ovarian failure. The gene discussed is IFNG; the disease is primary ovarian failure.