Chen et al., 2011). An animal study decrease insomnia-related anxiety (Wang et al., 2014). Furthermore, the inhibitory modulation of NF-κB and NLRP3 inflammasome activation by Wen-dan-tang may mediate its antidepressant effect (Jia et al., 2018). Overall, gastroesophageal reflux disease was reported to be associated with IBS (Ruigómez et al., 2009; Yarandi et al., 2010; de Bortoli et al., 2018). Wen-dan-tang consistently demonstrated significant improvement in symptom relief, and this efficacy was sustained over time in gastroesophageal reflux disease (Ling et al., 2015). This evidence concerns the gene NFKB1 and gastroesophageal reflux disease.