These outcomes revealed that miR-154-5p upregulation led to reduced weight and volume of tumors in nude mice and decreased CUL2 expression, while its downregulation improved CUL2 protein level but had no obvious influence on tumor weight and volume and CUL2 mRNA level, which demonstrated that miR-154-5p inhibited CUL2 translation via silencing CUL2 in vivo, thereby hindering the progression of cervical cancer. This evidence concerns the gene CUL2 and cervical cancer.