The dual luciferase reporter assay confirmed the targeted regulation effect of miR-154-5p and CUL2, and preliminarily discussed the anti-cancer effect role of miR-154-5p mediated CUL2 in HPV16-positive cervical cancer cells using transient transfection method (Zhao et al., 2020). The gene discussed is CUL2; the disease is cervical carcinoma.