One dog was excluded because he was blind in his left eye and had severe cachexia, one dog was excluded for having antibiotic resistant urinary tract infection, three dogs were excluded for abnormalities in their bloodwork; one had marked liver enzymes (alkaline phosphate, alanine transaminase and aspartate aminotransferase) elevation, one had leukocytosis, and was therefore tested for infectious disease and was positive for Rocky Mountain Spotted Fever and the other one showed electrolyte imbalances (hypocalcemia, hypomagnesemia and hyperkalemia), and had also a history of seizures. The gene discussed is GPT; the disease is Hypocalcemia.