Secondly, we measured the levels of pyroptosis pathway proteins by western blotting, and we found that the levels of NLRP3, ASC, and caspase-1 were all significantly increased in septic AKI (all P<0.001), while SNG reduced the expression of these proteins (all P<0.001) (Figure 4B-F). The gene discussed is CASP1; the disease is acute kidney injury.