ANXA5 and telomere syndrome: Moreover, flow cytometry analysis of the cells with Annexin V and 7-aminoactinomycin D (7-AAD) showed that unlike STS causing Annexin V+/7-AAD− early apoptotic cells in a short treatment, the EC330/EC359-treated cells directly became Annexin V+/7-AAD+, suggesting an early rupture of the cell membrane that allowed 7-AAD permeating into the cells (Fig. 1E).