While the transcription of representative HSV-1 viral genes of three classes was significantly lower in Oct-1 KO cells than in HEp-2 cells due to the lack of functional Oct-1 and VIC (Figure 2F), it is worth noting that the viruses from Oct-1 KO cells, cell-associated or secreted, transcribed their genomes less effectively than viruses from HEp-2 cells during the next round of infection in HEp-2 cells (Figures 2F,G). Here, POU2F1 is linked to infection.