Suppression of PRSS3 due to promoter methylation has been observed by several groups,[56, 57, 58] while more studies have reported the pro‐cancer role of PRSS3, especially its pro‐metastatic role.[59, 60, 61] In our study, we found that PRSS3 could enhance the invasion and metastatic abilities of cancer cells and was upregulated in human lung tumor tissues. This evidence concerns the gene PRSS3 and cancer.