At 10 days after transfection, CRISPRi-HML-2–transfected GBM cells had significantly reduced neurosphere formation compared with the no-guide CRISPRi–transfected (ngCRISPRi-transfected) cells, supporting the role of HML-2 transcription in cellular proliferation (Figure 7A). Here, CLEC10A is linked to glioblastoma.