CTSD and Wilson disease: However, in WD/CCl4-induced fibrotic livers, in addition to the small spots with low fluorescence intensity, we found large ring-like aggregates of CTSB and CTSD with high immunofluorescence signals deposited in fibrotic lesions; these ring-like aggregates was larger in Prep-/- mice than in Prep+/- mice (Fig. 7d, e).