To elucidate the role of Ly6d in nonparenchymal cells of the liver during NAFLD pathogenesis, we performed scRNA-seq on hepatocytes and nonparenchymal liver cells from HFD-fed control mice (CON, n = 2) and HFD-fed Ly6d KD mice (KD, n = 2). Here, LY6D is linked to metabolic dysfunction-associated steatotic liver disease.