TRPA1 and cancer: Immunoblots identified a major band of ∼140 kDa in both primary cultures of mCRC and control cells isolated from adjacent non-neoplastic tissue (Fig. 1A and Fig. S1), as also observed in other cancer cell types [20, 21], and densitometric analysis revealed that TRPA1 protein was significantly (p < 0.05) up-regulated in mCRC cells (Fig. 1B).