To evaluate potential effects of tTF-NGR on the expression of granzyme B and/or on hypoxia-inducible factor (HIF)-1α expression on CD8 + CAR T cells infiltrating tumor xenografts, we costained xenografts obtained at autopsy from the above in vivo experiments with antibodies against human CD8 and granzyme B or used RNAish for detection of HIF-1α, an early marker for hypoxia, along with DAPI. Here, GZMB is linked to neoplasm.