Our data confirmed that decreased pro-inflammatory cytokines (IL-2, IFN-γ, and IL-12) and elevated anti-inflammatory cytokines (IL-4, IL-10) in tumor-beared RNF8−/− mice, yet inhibition gal-3 treatment effectively increased pro-inflammatory cytokines and decreased anti-inflammatory cytokines infiltration in TME (Supplementary Fig. 6). This evidence concerns the gene RNF8 and neoplasm.