Then, the 8 LGG samples and 4 GBM samples were stained with TRAF6 or IRAK1 at the continuously adjacent position, and the positive scores (defined by the fluorescence intensities and ratio of positive cells) of TRAF6 and IRAK1 in the GBM group were higher than those in the LGG group. Here, TRAF6 is linked to glioblastoma.