At present, there are few studies on the biological expression of breast cancer at BI-RADS grades 3 to 5, and many studies have focused on a single ultrasound indicator.[21,22] In the present study, the expression levels of ER, PR and HER-2 were positively correlated with BI-RADS grades 3, 4 and 5. This evidence concerns the gene ESR1 and breast cancer.